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PodocinR138Q has a short half-life. Immunoblot analysis of the time course of podocin degradation after inhibition of protein synthesis with cycloheximide (CHX) (25 μm) in podocyte cell lines. Densitometry data from three independent experiments are represented as mean ± S.D. Half-lives were estimated by fitting a one-phase exponential decay curve to the data, as in the graph shown for ng-Podwt and ng-PodR138Q, and are summarized in the table.
