Fig. 3. Norbornene modification of 106 nt long model RNA. (A) Scheme for the post-transcriptional modification of capped 106 nt long RNA. (B) Post-transcriptional modification of a 106 nt capped model RNA. The model RNA was gel-purified after transcription and capped using the vaccinia capping system (but omitting AdoMet). A norbornene moiety was enzymatically appended followed by reaction with 11 to give a fluorescently labeled RNA, followed by separation on a 10% denat. PAGE (run for 1 h at 10 W) and scanned for TAMRA fluorescence on a Typhoon FLA9500 laser scanner. RNA was visualized by staining in SYBR Gold (Invitrogen) solution. M: Low Range RNA ladder (NEB).
