Fig. 2. Subcellular localization of MGH in living cells. (a) Confocal fluorescence microscopic images of MGH localization in HEK293T cells transfected with a plasmid encoding Halo-NLS (nuclei), Lyn₁₁-Halo (inner leaflet of cell membrane) or HaloTag (cytoplasm). Labeling reactions of fusion protein of Halo-NLS, Lyn₁₁-Halo or HaloTag in HEK293T cells with 3 μM MGH(AM) and staining with 200 ng mL^–1 Hoechst 33342 were performed for 1 h at 37 °C. (b) Confocal fluorescence microscopic images of MGH localization in ER or mitochondria matrix, which are surrounded by a lipid bilayer membrane. HEK293T cells transiently expressing Halo-ER or 2xCOX8-Halo were incubated with 3 μM MGH(AM) and either 200 nM ER-Tracker Red or 200 nM MitoTracker DeepRed for 1 h at 37 °C. Scale bar: 20 μm.