Figure 5.

Effects of paeonol on microglial activation at 72 h and 28 days after reperfusion in rats. (A) Representative photomicrographs displaying Iba-1-immunopositive microglia in the ischemic core and boundary zone regions at 72 h and 28 days after reperfusion (scale bar, 100 µm) and (B-E) quantification results. At 72 h after reperfusion, Iba-1-positive microglia initially increased in the ischemic core and the boundary zone regions, and these changes were significantly inhibited by paeonol treatment. At 28 days after reperfusion, microglial cells in the ischemic core and boundary zone regions were significantly increased, which was attenuated by paeonol treatment. Values are expressed as the mean ± standard error of the mean from eight rats per group. **P<0.01 compared with sham group, ^##P<0.01 compared with model group. Pae, paeonol; MCAO, middle cerebral artery occlusion; Iba-1, ionized calcium binding adaptor molecule 1.