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. 2017 Sep 1;20(2):200–204. doi: 10.4103/aja.aja_30_17

Figure 4.

Figure 4

ROS generation induced by Andro in PC3 cells. (a) ROS generation in PC3 cells treated with Andro (20 μmol l−1) and TRAIL (20 ng ml−1) or both for 12 h detected using flow cytometry. PC3 cells were pretreated with or without 5 mmol l−1 NAC for 30 min and then treated with or without Andro (20 μmol l−1) for 12 h, detection of (b) ROS generation using flow cytometry, (c) and the expression of p53 and DR4 using western blot analysis. PC3 cells were pretreated with or without 5 mmol l−1 NAC for 30 min and then treated with or without both TRAIL (20 ng ml−1) and Andro (20 μmol l−1), detection of (d) the expressions of PARP and caspase-3, and (e) the apoptosis of PC3 cells. NAC vs control, *P < 0.05. Andro: andrographolide; TRAIL: tumor necrosis factor-related apoptosis-inducing ligand; ROS: reactive oxygen species; NAC: N-acetyl-cysteine.