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. 2018 Mar 19;11:71. doi: 10.1186/s13068-018-1075-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — HPAEC-PAD chromatograms of enzyme reaction products and LRP alkali. Comparison of enzyme reaction products released from LRP by CuGE, GH10 endo-xylanase or both from reactions with 5 mg/mL substrate. The chromatograms show a trace of neutral xylo-oligosaccharides from DP1 to DP6 (black) and the NaOH extracted and precipitated glucuronoxylan fraction from LRP as it appears before enzymatic hydrolysis (light blue, LRP alkali, from 37.5 mg/mL substrate). The order of elution on HPAEC is so that neutral components elute first with increasing DP, whereas charged components are retained longer and in this case start to elute after approximately 15 min