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Probe sequences targeting six mutation types
| Genes | Mutations | Probe sequences (5'⟶3') |
|---|---|---|
| BRAF exon 15 | 1799T>A | R6G-CTAGCTACAGAGAAATCTCG |
| KRAS exon 2 | 34G>T | Cy3-TTGGAGCTTGTGGCGTAGGCA |
| 35G>A | Cy5-TTGGAGCTGATGGCGTAGGCA | |
| 35G>T | TAMRA-TTGGAGCTGTTGGCGTAGGCA | |
| 38G>A | ROX-TTGGAGCTGGTGACGTAGGCA | |
| PIK3CA exon 9 | 1624G>A | FAM-ATCCTCTCTCTAAAATCACTGAGC |
