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. Author manuscript; available in PMC: 2018 Nov 16.
Published in final edited form as: Biochem J. 2017 Nov 16;474(23):3903–3914. doi: 10.1042/BCJ20170279

Figure 5. CA-Rac1 stimulates macropinocytosis.

Figure 5

(A) Stable p110β knockdown cells were transfected with wild-type p110β and GFP-CA-Rac1. After 48 h, cells were pretreated with 0.1% DMSO or 25 μM EIPA for 90 min, incubated with 1 mg/ml 70 kDa rhodamine-Dextran for 30 min at 37°C, fixed and imaged. (B) Stable p110β knockdown cells were transfected with wild-type p110β or p110βRBD, with or without GFP-CA-Rac1. The number of macropinosomes per cell (defined as vesicles greater than 0.75 μm in diameter) in GFP-positive cells were counted as described in the Experimental Procedures. Scale bar = 20 μm.