Figure 3.

Cordyceps militaris fraction induces G2/M phase arrest and regulates the expression of cell cycle-related mitotic proteins in KB cells. (a) Effect of Cordyceps militaris fraction on cell cycle distribution of KB cells. After treatment of cells with Cordyceps militaris fraction, the cell cycle proportions were detected by flow cytometry using propidium iodide staining. (b) Histograms of cell cycle distribution in nontreated and treated KB cells. Each value represents the mean ± standard deviation of three independent experiments. *P < 0.05 and **P < 0.01, compared to the control group. (c and d) Effect of Cordyceps militaris fraction on the expression of cell cycle-related mitotic proteins in KB cells. Cells were treated with the indicated concentrations of Cordyceps militaris fraction for 8 h, and cell lysates were analyzed by Western blot analysis