Figure 4.

Cordyceps militaris fraction induces activation of c-Jun N-terminal kinase and c-Jun in KB cells. (a and b) Effect of Cordyceps militaris fraction on the expression of phosphor mitogen-activated protein kinases and p-c-Jun in KB cells. Cells were treated with 50 μg/ml of Cordyceps militaris fraction for indicated hours (a) or with indicated concentrations of Cordyceps militaris fraction for 8 h (b), and cell lysates were analyzed by Western blot analysis. (c) Effect of p-38 inhibitor SB203580 on Cordyceps militaris fraction-regulated expression of p-p38 in KB cells. Cells were treated with 20 μM SB203580 for 1 h prior to exposure to 50 μg/ml of Cordyceps militaris fraction for 6 h, and cell lysates were analyzed by Western blot analysis. (d) Effect of c-Jun N-terminal kinase inhibitor SP600125 on Cordyceps militaris fraction-regulated expression of p-c-Jun N-terminal kinase in KB cells. Cells were treated with 20 μM SP600125 for 1 h prior to exposure to 50 μg/ml of Cordyceps militaris fraction for 6 h, and cell lysates were analyzed by Western blot analysis. (e) Effects of c-Jun N-terminal kinase inhibitor SP600125 and p-38 inhibitor SB203580 on Cordyceps militaris fraction-regulated expression of p-c-Jun in KB cells. Cells were treated with 20 μM SP600125 or 20 μM SB203580 for 1 h prior to exposure to 50 μg/ml of Cordyceps militaris fraction for 6 h, and cell lysates were analyzed by Western blot analysis