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. 2018 Feb 20;14(53):116–123. doi: 10.4103/pm.pm_63_17

Figure 6.

Figure 6

Cordyceps militaris fraction induces apoptosis and cell cycle arrest in a manner dependent on c-Jun N-terminal kinase/c-Jun signaling pathway. (a) Effect of c-Jun N-terminal kinase inhibitor SP600125 on Cordyceps militaris fraction-induced apoptosis in KB cells. Cells were treated with 20 μg/ml of Cordyceps militaris fraction alone or in combination with 20 μM SP600125 for 48 h, and then Annexin V-FITC/PI double-staining analysis was performed. (b) Effect of c-Jun N-terminal kinase inhibitor SP600125 on Cordyceps militaris fraction-induced G2/M arrest in KB cells. Cells were treated with 20 μg/ml of Cordyceps militaris fraction alone or in combination with 20 μM SP600125 for 24 h, and then PI staining analysis was performed. (c) Effect of c-Jun N-terminal kinase inhibitor SP600125 on Cordyceps militaris fraction-regulated expression of caspase-9 and PARP in KB cells. Cells were cotreated with 20 μg/ml of Cordyceps militaris fraction and 20 μM SP600125, and cell lysates were analyzed by Western blot analysis. (d) Effect of c-Jun N-terminal kinase inhibitor SP600125 on Cordyceps militaris fraction-regulated expression of mitotic cyclin/kinase/phosphatase-related proteins in KB cells. Cells were cotreated with 20 μg/ml of Cordyceps militaris fraction and 20 μM SP600125, and cell lysates were analyzed by Western blot analysis