Figure 2.

Panel (A), dose depend cytotoxicity effect of PAMAM dendrimer in J774A.1; cytotoxicity assay was performed via alamar blue (AB) and MTT assay. The concentration ranges used for the cytotoxicity assays with G4, G5, and G6 were 0.08 μM to 6 μM; 0.03 μM to 2 μM; and 0.013 μM to 1 μM respectively, as determined from an initial range finding study. Panel (B), Confocal laser scanning micrographs of intracellular ROS generation in J774A.1 cells, following exposure to negative control, positive control (H₂O₂), PAMAM G-4, PAMAM G-5, and PAMAM G-6. The data are shown after a 2 h exposure of PAMAM dendrimers. Panel (C), graphical representation of different responses as a function of time. Figure reproduced with permission from reference [45].