Skip to main content
. 2018 Mar;59(3):1454–1466. doi: 10.1167/iovs.17-21964

Figure 1.

Figure 1

Wnt signaling activation increased membrane-associated β-catenin and total cadherin in primary NTM cells. Primary NTM cells were treated with or without Wnt3a and/or sFRP1 for 24 hours in serum-free medium before fixation and ICF. (A) NTM cells were stained for β-catenin (green), F-actin (red), and nuclei (blue) using antibody, phalloidin-Alexa 594 and DAPI, respectively. White arrows indicate filopodia-like cell-cell connections enriched with membrane-associated β-catenin. (B) ICF staining of total cadherins (green) and nuclei (blue).