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Wnt signaling and K-cadherin regulated HTM cell impedence. Maximum and minimum normalized CI values of NTM cells were collected over 72 hours using the Acea RTCA iCelligence system. (A) NTM cells were treated with or without Wnt3a and/or sFRP1. (B) NTM cells were transfected with 1nM nontargeting (NT), anti-OB-cadherin, or anti-K-cadherin siRNA. (C) siRNA transfected NTM cells were used for WB to determine protein expression levels.
