Figure 4.

Effect of CX-01 on U937 cell migration toward CXCL12. The assay was conducted using a cell migration assay kit from Cell Biolabs (San Diego, CA), as detailed in the Methods section. CXCL12 was added at a concentration of 100 ng/mL in the wells. CX-01 was added at a concentration of 200 µg/mL in the wells. To investigate the direct effect of CX-01 on leukemia cells in 1 variable, CXCL12 only was added to the wells and U937 cells were pretreated for 30 minutes with 500 µg/mL CX-01 before loading them into inserts. The graph shows averages and standard errors of the mean of 2 to 3 replicate wells in each group (the media blank variable had 1 well). The legend indicates CXCL12 and/or CX-01 added to the wells or pretreatment of U937 cells with CX-01. The graph is representative of 2 separate experiments. RFU, relative fluorescence unit.