Table 2.
Representative DRB1*01:01-restricted T-cell clones recognizing FVIII2194-2213
| T-cell clone | TCRBV* | TCRBD* | TCRBJ* | No. of clones† |
|---|---|---|---|---|
| 17A-19WK-11 | 20-1‡ | 2*01 or *02 | 2-7*01 | 3 |
| 17A-21MO-5 | 6-6*01 or *03 | 1*01 | 2-2*01 | 5 |
| 17A-21MO-11 | 5-1*01 | 1*01 | 1-2*01 | 3 |
| 32A-18 | 6-1*01 | 1*01 | 2-7*01 | 6 |
| 56A-C2 | 27*01 | Not identified | 1-1*01 | 10 |
Five CD4+ T-cell clones having unique TCRB sequences were chosen from 27 clones isolated from subjects GS1-17A, GS1-32A, and GS1-56A.17 These clones all bound with medium or high avidity to a DRB1*01:01 tetramer loaded with FVIII2194-2213, expanded well in culture, and proliferated in response to FVIII2194-2213. One clone for each unique TCRB sequence was selected to evaluate immune responses to FVIII peptides and proteins.
*
TCR nomenclature is in accordance with the international reference IMGT/GENE-DB.70
†
The total number of clones isolated with this TCRB gene.
‡
Sequence was identical to 20-1*01, *02, *04, *05, and *06 alleles.