Skip to main content
. 2018 Jan 8;9(4):674–682. doi: 10.7150/jca.22277

Figure 2.

Figure 2

Digestion of plasmid dFv-LDP, dFv-R-LDP and its protein expression in E.coli. A: agarose gel electrophoresis of digested plasmid dFv-LDP and dFv-R-LDP. Lane 1 and 3, plasmid dFv-LDP and dFv-R-LDP were digested with Nde I/Xho I, respectively; Lane 2 and 4, plasmid dFv-LDP and dFv-R-LDP were digested with Hind III/Xho I, respectively; Lane 5, the plasmid pET30 a(+); Ma and Mb were the DNA marker DL2000 and DL15000, respectively. B: the SDS-PAGE analysis of purified protein dFv-LDP (lane 1) and dFv-R-LDP (lane 2), the molecule weight was about 65 kDa.