Skip to main content
. 2018 Feb 12;8(7):1766–1781. doi: 10.7150/thno.22788

Figure 6.

Figure 6

BM Sca-1+ cells activated EMT of EPDCs through TGF-β1 signaling. Epicardial-derived cells (EPDCs, abbreviated as E) were separately co-cultured with Sca-1- cells (S-), Sca-1+ cells (S+), TGF-β1 (T, 5 ng/mL) or Sca-1+ cells with a TGF-β1 blocking antibody (S++BL, 1 µg/mL) under hypoxia (0.1% O2) conditions for 72 h. Young Sca1+ or Sca1- BM cells (1×105/cm2) in serum-free DMEM medium were plated in the transwell cell culture inserts (1 μm diameter pores). EPDCs (2×104/cm2) were cultured in the lower compartment. EMT transcriptional genes (A), epithelial genes (B) and mesenchymal genes (C) in EPDCs were quantified by RT-qPCR. (D) Immunofluorescent staining of mesenchymal markers (smooth muscle actin, vimentin, calponin) and epithelial tight junction protein (ZO-1) in EPDCs. (E) The protein levels of vimentin and calponin in EPDCs were determined by Western blot and normalized by GAPDH. n=6/group, mean ± SD; **P<0.01.