Skip to main content
. 2018 Feb 12;8(7):1808–1823. doi: 10.7150/thno.23177

Figure 2.

Figure 2

PHGDH depletion exerts preferential toxicity in erlotinib resistant cells. (A) Knockdown efficiency of PHGDH by siPHGDH candidates was measured by immunoblotting. (B-E) Clonogenic assays were performed after cells transfected with 20 nM siPHGDHs as indicated. (F, G) Cells were transfected with 25 nM siPHGDH#4 for 72h, apoptotic cells were analyzed by annexinV-PI double staining assay. (H) Cell energy phenotype test. Knockdown of PHGDH restrained both ECAR and OCR in the PC9ER4 cells after 25 nM siPHGDHs transfection for 72 h. Error bars represent mean ± SEM of triplicates. *, p < 0.05; ****, p < 0.0001.