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. 2018 Feb 12;8(7):1808–1823. doi: 10.7150/thno.23177

Figure 3.

Figure 3

PHGDH inhibition re-sensitizes the erlotinib resistant NSCLC cells to erlotinib treatment. (A) PC9ER4 cells was transfected with various concentrations of siPHGDH#4 for 72h. (B) PC9ER4 cells were treated with different doses of erlotinib and indicated siPHGDHs or negative siRNA (mock) at 5 nM for 72h. PC9 cells were exposed to erlotinib with transfection medium as control. (C) HCC827ER9 cells were co-treated with different doses of erlotinib and siPHGDH#4 (10 nM) or siPHGDH#5 (20 nM) for 72 h. HCC827 cells were exposed to erlotinib with transfection medium as control. (D) NCT-503, a PHGDH inhibitor, treated indicated cells for 72 h. (E and F) PC9ER4 and HCC827ER9 were co-treated with NCT-503 and erlotinib at various dosages for 72 h. The cell growth inhibition rate was analyzed by CCK8 assays after the treatment described above. Results were shown as mean ± SEM of triplicates. *, p < 0.05; **, p < 0.01.