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(a) Hemolysis assay of TPIP-FONs at several concentrations on human RBCs for 30 min at 37 °C. The mixture was centrifuged to detect the cell-free hemoglobin in the supernatant. RBCs incubated with PBS and water were used as negative (-) and positive (+) controls, respectively. (b) Histological evaluation of different organs (heart, liver, spleen, lung, and kidney) from rats treated with PBS and TPIP-FONs.
