Skip to main content
. 2018 Feb 15;8(7):1940–1955. doi: 10.7150/thno.23674

Figure 5.

Figure 5

The stimulatory effect of Sr and/or Si ions on chondrocyte maturation. (A) HIF-1α gene, (B) COL II gene, (C) Aggrecan gene, (D) SOX9 gene, (E) N-cadh gene. Sr ions increased chondrocyte specified gene expression through activating HIF-1α pathway within the concentration range of 0.25-4 mg/L. Furthermore, Sr and Si ions markedly enhanced COL II, Aggrecan, SOX9, N-cadh expression within the concentration range of Sr ions 0.25-4 mg/L and Si ions 0.5-8 mg/L, respectively. In addition, the expression of COL II gene was up-regulated by Sr and Si ions together at the concentration of Sr ions 0.25 mg/L and Si ions 0.5 mg/L. The expression level of Aggrecan gene was stimulated by Sr and Si ions cooperatively within the concentration range of Sr ions 1-4 mg/L and Si ions 2-8 mg/L, and the expression of SOX9 gene was elevated by Sr and Si ions at the concentration of Sr ions 4 mg/L and Si ions 8 mg/L. Also, N-cadh gene was enhanced by Sr and Si ions cooperatively within the concentration range of Sr ions 0.25-2 mg/L and Si ions 0.5-4 mg/L. The relative gene amount of CTR groups was set as 1, the value of expression gene was the fold change relative to CTR.Repeat number: n=3. (*p<0.05, **p<0.01, ***p<0.001).