Figure 10.

Non-cell-autonomous regulation by Vgat mutation of excitatory synapses onto PV⁺ErbB4⁺ interneurons. A, Paradigm of low-dose Tam administration and experimental timeline. B, Representative coronal sections showing sparsely recombined ErbB4⁺ interneurons (red, labeled by tdTomato) and PV⁺ interneurons (magenta). Higher-magnification image of the dotted rectangle (B′) is shown on the right. C, No change in excitatory synapses onto sparsely recombined PV⁺ErbB4⁺ interneurons compared with PV⁺ interneurons without recombination. Cortical sections were stained with antibodies against PV and VGLUT-1. Shown are L4 interneurons in which ErbB4⁺ interneurons are labeled in red (tdTomato), VGLUT-1 punctae in green, and PV⁺ interneurons in magenta. D, Quantitative data in C. n = 21 PV⁺ and n = 19 PV⁺ErbB4⁺ interneurons, 3 mice. n.s., Not significant. Error bars indicate SEM.