Fig. 3.

IL10 blocks and reverses cilia elongation upon inflammatory cytokine stimulation in mouse and human endothelial cells. (a) MEC were either incubated with IL10 (10 ng/mL), TNFα (10 ng/mL), or left untreated for 12 h. Subsequently, different combinations of TNF or IL10 were added directly into the previous conditions and incubated for another 12 h (n ≥ 26 cilia per condition). (b) The combination treatments were repeated in human pulmonary MVEC of healthy individuals (donor = 3, n ≥ 28 cilia per condition). (c) MIP of representative cilia (arrowheads) on MVEC under the different conditions.