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. 2018 Mar 16;6:e4473. doi: 10.7717/peerj.4473

Figure 1. Amplification efficiency and standard curves of c-myc and GAPDH.

Figure 1

Seven different dilutions of RNA were used as an input to synthesize cDNA, then to a real-time quantitative PCR reaction with c-myc and GAPDH primers. (A) ΔCT values were calculated by subtracting the CT values from three independent samples of the control gene(GAPDH) from the target c-myc. Averages and standard deviations are shown as points and error bars. The blue line represents the linear trend between the ΔCT and log10 of the input amount. (B) CT values from three independent samples of c-myc and GAPDH are shown with the corresponding log10 input amounts.