Table 1. Average CT value for c-myc and GAPDH at different input amounts.
Input RNA (ng) | c-myc average CT | GAPDH average CT | ΔCT c-myc-GAPDH |
---|---|---|---|
1.0 | 25.59 ± 0.04 | 22.64 ± 0.03 | 2.95 ± 0.05 |
0.5 | 26.77 ± 0.09 | 23.73 ± 0.05 | 3.04 ± 0.10 |
0.2 | 28.14 ± 0.05 | 25.12 ± 0.10 | 3.02 ± 0.11 |
0.1 | 29.18 ± 0.13 | 26.16 ± 0.02 | 3.01 ± 0.13 |
0.05 | 30.14 ± 0.03 | 27.17 ± 0.06 | 2.97 ± 0.07 |
0.02 | 31.44 ± 0.16 | 28.62 ± 0.10 | 2.82 ± 0.19 |
0.02 | 32.42 ± 0.12 | 29.45 ± 0.08 | 2.97 ± 0.14 |