Table 2. Relative quantification using comparative (ΔΔC_T) method (separate tubes).

Tissue	c-myc CT	GAPDH CT	ΔCT c-myc-GAPDH	ΔΔCTΔCT − ΔCT,Brain	c-mycN Rel. to Brain
Brain	30.72	23.7
	30.34	23.56
	30.58	23.47
	30.34	23.65
	30.5	23.69
	30.43	23.68
Average	30.49 ± 0.15	23.63 ± 0.09	6.86 ± 0.17	0.00 ± 0.17	1.0 (0.9–1.1)
Kidney	27.06	22.76
	27.03	22.61
	27.03	22.62
	27.1	22.6
	26.99	22.61
	26.94	24.18
Average	27.03 ± 0.06	22.66 ± 0.08	4.37 ± 0.10	−2.50 ± 0.10	5.6 (5.3–6.0)