Table 3. Relative quantification using the standard curve method (separate tube).
Tissue | c-myc (ng) | GAPDH (ng) | c-mycN norm. to GAPDH | c-mycN Rel. to Brain |
---|---|---|---|---|
Brain | 0.033 | 0.51 | ||
0.043 | 0.56 | |||
0.036 | 0.59 | |||
0.043 | 0.53 | |||
0.039 | 0.51 | |||
0.040 | 0.52 | |||
Average | 0.039 ± 0.004 | 0.54 ± 0.034 | 0.07 ± 0.008 | 1.0 ± 0.12 |
Kidney | 0.40 | 0.96 | ||
0.41 | 1.06 | |||
0.41 | 1.05 | |||
0.39 | 1.07 | |||
0.42 | 1.06 | |||
0.43 | 0.96 | |||
Average | 0.41 ± 0.016 | 1.02 ± 0.052 | 0.40 ± 0.025 | 5.5 ± 0.35 |