Table 1. PCR primers.
Gene | Protein | Accession | Primers |
---|---|---|---|
KCa3.1 | Calcium-activated potassium channel 3.1 | NM_002250.2 |
F: GCCCTGGAGAAACAGATTGA R: CATAGCAGCATAGTGAGAGTG |
α-SMA | α-smooth muscle actin | NM_001613 |
F: TGGGTGACGAAGCACAGAGC R: CTTCAGGGGCAACACGAAGC |
Col1A1 | Collagen I | NM_000088.3 |
F: TGTGGCCCAGAAGAACTGGTACAT R: ACTGGAATCCATCGGTCATGCTCT |
ß-actin | ß-actin | X00351.1 |
F: AGGCCAACCGCGAGAAGATGACC R: GAAGTCCAGGGCGACGTAGCAC |
α-SMA | α-smooth muscle actin | NM_007392.3 |
F: GGGAGTAATGGTTGGAATGG R: GATGATGCCGTGTTCTATCG |
Col1A1 | Collagen I | NM_007742.4 |
F: CGGTTATGACTTCAGCTTCC R: CGAACCACGTTAGCATCATC |
Col4A1 | Collagen IV | NM_009931.2 |
F: GGCATTGTGGAGTGTCAA R: CCTTTCTGACCTTTCTGTCC |
TGFβ1 | Transforming growth factor-β1 | NM_011577 |
F: CTACCATGCCAACTTCTGTC R: GGGTTGTGTTGGTTGTAGAG |
ß-actin | ß-actin | NM_007393.3 |
F: TGGCTACAGCTTCACCACCA R: GAAGAGCTATGAGCTGCCTG |