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. 2018 Mar 19;13(3):e0194667. doi: 10.1371/journal.pone.0194667

Fig 3. Expression of proinflammatory mediators in IMQ-treated skin and in cultured skin cells of Il-38 deficient mice.

Fig 3

Il-38-/- mice and WT littermates were treated daily with a topical dose of 12.5mg of Aldara™ cream (0.625mg IMQ), for 7 days. Skin mRNA levels for Il-36α, Il-36β, Il-36γ, Il-36ra, Il-38, Cxcl-1 and Il-6 were quantified by real-time RT-qPCR on day 7 in the IMQ-treated ears (A). Data were expressed relative to L32 levels. Results represent individual values and mean ± SEM of n = 5 mice per group. Statistical analysis was performed by unpaired Mann-Whitney comparison test. A p-value < 0.05 was considered significant. ** p<0.01. Cultured primary keratinocytes (Kera) and dermal fibroblasts (Fibro) isolated from naïve Il-38-/- (dark symbols) or WT mice (white symbols), were stimulated with rec. mouse Il-36β at 100ng/ml for 6 h, or left unstimulated (Med). Il-36α, Il-36β, Il-36γ, Il-36ra, Il-38, Cxcl-1, Il-6 and S100a9 mRNA levels were quantified by real-time RT-qPCR (B). Data are expressed relative to L32 levels. Results represent individual values and mean ± SEM of n = 6–9 biological replicates per group. Statistical analysis was performed by two-way ANOVA followed by a Holm–Sidak’s comparison test. A p-value < 0.05 was considered significant. *** p<0.001, ** p<0.01, * p<0.05.