Table 1. Primers used for qPCR.
| Gene | Accession number | Primer sequence | Amplicon (pb) | |
|---|---|---|---|---|
| Col1a1 | NM_007742.4 |
Fwd 5’-GGCTCCTGCTCCTCTTAG-3’ Rev 5’-ACAGTCCAGTTCTTCATTGC-3’ |
194 | |
| Cxcl1 | NM_008176.3 |
Fwd 5'-ACTCAAGAATGGTCGCGAGG-3' Rev 5'-GTGCCATCAGAGCAGTCTGT-3’ |
123 | |
| K14 | Var1 | NM_016958.2 |
Fwd 5’-ATCGAGGACCTGAAGAGCAA-3’ Rev 5’-GGCTCTCAATCTGCATCTCC-3’ |
220 |
| Var2 | NM_001313956.1 | |||
| Il-1a | NM_010554.4 |
Fwd 5'-GGGAAGATTCTGAAGAAGAG-3' Rev 5'-GAGTAACAGGATATTTAGAGTCG-3’ |
319 | |
| Il-1b | NM_008361.4 |
Fwd 5'-TGTGAAATGCCACCTTTTGA-3' Rev 5'-GTGCTCATGTCCTCATCCTG-3’ |
248 | |
| Il-6 | NM_031168.2 |
Fwd 5'-TGAACAACGATGATGCACTTGCAGA-3' Rev 5'-TCTGTATCTCTCTGAAGGACTCTGGCT-3’ |
211 | |
| Il-18 | NM_008360.1 |
Fwd 5'-CAGGCCTGACATCTTCTG-3' Rev 5'-CTGACATGGCAGCCATT-3’ |
104 | |
| Il-36a | NM_019450.3 |
Fwd 5'-TAGTGGGTGTAGTTCTGTAGTGTGC-3' Rev 5'-GTTCGTTCAAGAGTGTCCAGATAT-3’ |
268 | |
| Il-36b | NM_027163.4 |
Fwd 5'-ACAAAAAGCCTTTCTGTTCTATCAT-3' Rev 5'-CCATGTTGGATTTACTTCTCAGACT-3’ |
186 | |
| Il-36g | NM_153511.3 |
Fwd 5'-AGAGTAACCCCAGTCAGCGTG-3' Rev 5'-AGGGTGGTGGTACAAATCCAA-3’ |
186 | |
| Il-36r | NM_133193.3 |
Fwd 5'-AAACACCTAGCAAAAGCCCAG-3' Rev 5'-AGACTGCCCGATTTTCCTATG-3’ |
262 | |
| Il-36ra | NM_019451.2 |
Fwd 5'-TGGAGCTCATGATGGTTCTG-3' Rev 5'-TAATGACCTTCTCTGCGTGC-3’ |
123 | |
| Il-38 | NM_153077.2 |
Fwd 5'-CCTGGCGTGTGTAAAGACAA-3' Rev 5'-CAGATCCCAAGCTTCTCTGG-3’ |
125 | |
| Rpl32 | NM_172086.2 |
Fwd 5'-CACCAGTCAGACCGATATGTGAAAA-3' Rev 5'-TGTTGTCAATGCCTCTGGGTTT-3' |
64 | |
| S100a9 | Var1 | NM_001281852.1 |
Fwd 5'-CACCCTGAGCAAGAAGGAAT-3' Rev 5'-TGTCATTTATGAGGGCTTCATTT-3' |
95 |
| Var2 | NM_009114.3 | |||
| Tnfa | NM_013693.3 |
Fwd 5'-AGTTCTATGGCCCAGACCCT-3' Rev 5'-GTCTTTGAGATCCATGCCGT-3’ |
159 | |
| Vim | NM_011701.4 |
Fwd 5’-CGGCTGCGAGAGAAATTGC -3’ Rev 5’-CCACTTTCCGTTCAAGGTCAAG-3’ |
124 | |