Figure 7. Classification of in vivo scaffold organization and corresponding migration modes in the B16F10 model. Both, connective tissue composed of predominantly fibrillar collagen or surrounding cell structures may show random or aligned organization, with impact on the invasion mode of B16F10 cells. Blue, THG-/SHG-positive scaffold; red, basement membrane, not detected by MPM of native tissue structures; yellow, tumor cells. 3D topography corresponds to either 1D (migration along a single fiber) 2D topography (migration along a surface) and 3D (when the cell bodies are confined by additional tissue structures apically or laterally interfaces, or move between multiple collagen fibers).