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. Author manuscript; available in PMC: 2018 Mar 19.
Published in final edited form as: Stem Cell Res. 2018 Jan 4;27:46–56. doi: 10.1016/j.scr.2017.12.016

Fig. 2. PRDM14 expression in testicular and intracranial germ cell tumors.

Fig. 2

A. Western blot of nuclear (5 μg) and cytoplasmic (5 μg) extracts from embryonal carcinoma cell (ECC) lines, Ntera2 (NT) and GCT27. Blots probed for PRDM14 (64 kD), OCT4 (45 kD), and beta-actin (42 kD).

B. Immunofluorescence of ECC line, GCT27, showing staining for PRDM14/OCT4, top panel. Immunofluorescence of paraffin section of a seminoma tumor sample, showing staining for PRDM14/OCT4, bottom panel. Scale bars, 15 μm.

C. Immunohistochemistry of paraffin section of tumor sample containing corresponding GCNIS portion of the tumor, staining for PRDM14, and GCNIS markers OCT4 and PLAP. Scale bars, 15 μm.

D. Immunohistochemistry of paraffin section of mixed germ cell tumor, corresponding to embryonal carcinoma, staining for PRDM14, SOX2 and SOX17, top panel. Magnified images of cells demarked in yellow box in top panel. Scale bars, 15 μm (top panel). 5 μm (bottom panel).

E. Immunohistochemistry staining of intracranial germinoma, yolk sac and teratoma, for PRDM14. Scale bars, 20 μm.