Fig. 2.

The effects of Ad5-gsgAM immunization on AHR, inflammatory cell infiltration, and total serum IgE. One week after sensitization with saline or OVA, mice were immunized with saline, Ad5, BCG, or Ad5-gsgAM and then challenged with OVA for three consecutive days. Twenty-four hours after the final challenge, they were subjected to whole-body plethysmograph analysis followed by sacrifice and lavage. a The schedules of sensitization, immunization, challenge, and detection. b The AHR in response to increased doses of inhaled MCh. c The absolute numbers of total cells, eosinophils, macrophages, lymphocytes, and neutrophils in the BALFs were counted using H&E staining. d The percentages of total cells, eosinophils, macrophages, lymphocytes, and neutrophils in the BALFs. e The concentrations of total serum IgE before and after challenge were assessed by ELISA. Data are presented as the mean ± SEM (n = 5 mice per group). Representative results from one of three independent experiments are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance