Fig. 3.

The effects of Ad5-gsgAM immunization on airway inflammation and goblet cell hyperplasia. Twenty-four hours after the final challenge, mouse lung tissue sections were subjected to histological examination. a Representative photomicrographs of H&E-stained lung sections from each group of mice (upper left panel: Saline mice; upper middle panel: OVA mice; upper right panel: OVA/Ad5 mice; bottom left panel: OVA/BCG mice; bottom middle panel: OVA/gsgAM mice). Bar = 100 μm. b Quantitative assessment of the peribronchial and perivascular inflammation. c Representative photomicrographs of PAS-stained lung sections from each group of mice (upper left panel: Saline mice; upper middle panel: OVA mice; upper right panel: OVA/Ad5 mice; bottom left panel: OVA/BCG mice; bottom middle panel: OVA/gsgAM mice). Bar = 100 μm. d Quantitative assessment of goblet cell hyperplasia. Data are presented as the mean ± SEM (n = 5 mice per group). Representative results from one of three independent experiments are shown. *P < 0.05, ***P < 0.001