Fig. 5.

Profiling of cytokines in the BALFs and serums. Twenty-four hours after the final challenge, the cytokines in the BALFs and serums of different group of mice were evaluated by ELISA. a–f The concentrations of IFN-γ (a), IL-4(b), IL-5 (c), IL-13 (d), IL-10 (e), and TNF-α (f) in the BALFs. g–i The contents of IL-33 (g) and sST2 (f) in the serums were assessed by ELISA and the ratios (i) of sST2 to IL-33 were calculated and shown. Data are presented as the mean ± SEM (n = 5 mice per group). Representative results from one of three independent experiments are shown. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no significance