Table 1.
The virus titer in lungs of BALB/c mice infected with 6D2 influenza virus.
| Average titer (mean log10 TCID50 ± SD) |
||
|---|---|---|
| Virus | Day 3 | Day 6 |
| r6D2-WT | 1.22 ± 2.12 | 3.17 ± 2.75 |
| r6D2-MA (HA) | 0.79 ± 1.37 | 2.65 ± 1.06 |
| r6D2-MA (PA) | 0.83 ± 1.44 | 0.44 ± 0.77 |
| r6D2-MA (PB2) | 5.22 ± 1.17 | 4.56 ± 1.07 |
| r6D2-MA (PA/PB2) | 5.22 ± 0.96 | 3.08 ± 0.83a |
| r6D2-MA (HA/PA/PB2) | 8.07 ± 0.54∗∗ | 3.89 ± 0.59 |
Three BALB/c mice were inoculated with 106TCID50 virus in 50 μL. Three mice in each group were euthanized for lung virus titration on day 3 and day 6 post inoculation. Lungs of mice were collected, homogenized, and the supernatants were used for virus titration using TCID50 in MDCK cells. The titration of virus is presented with mean log10 TCID50 ± SD. Statistical difference was measured with one-way ANOVA, followed with a Bonferroni correction, ∗∗P < 0.01 [r6D2-MA (HA/PA/PB2) vs. r6D2-WT],a one of the three mice died.