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. 2018 Mar 19;8:4755. doi: 10.1038/s41598-018-23024-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagram of the T-DNA region of the plasmids pBYR2fp-EGFP, pBYR2HS-EGFP, pBYR2EE-EGFP, pBYR2HH-EGFP, pBYR2H-EGFP, pBYR2TN-EGFP, pBYR2HT-EGFP, pBYR2HTS-EGFP, and pBYR2T-EGFP. 35S-p x 2, CaMV 35 S promoter with double-enhanced element; AtADH5′, 5′-untranslated region (UTR) of Arabidopsis thaliana alcohol dehydrogenase gene; TMV Ω, 5′-leader sequence of tobacco mosaic virus; EGFP, enhanced green fluorescence protein; HSPter, terminator of heat shock protein gene; Ext3′, tobacco extension gene 3′ element; 35Ster, terminator of CaMV 35S; NOSter, NOS terminator; LIR, long intergenic region of bean yellow dwarf virus (BeYDV) genome; SIR, short intergenic region of BeYDV genome; C1/C2, BeYDV ORFs C1 and C2 encoding for replication initiation protein (Rep) and RepA, respectively; LB and RB, the left and right borders of the T-DNA region, respectively; Nos-p and Nos-t, NOS promoter and terminator, respectively; and p19, a gene-silencing suppressor gene from tomato bushy stunt virus.