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. 2018 Mar 19;8:4755. doi: 10.1038/s41598-018-23024-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of HSP terminator on transient GFP expression at 3 days post-agroinfiltration. Total soluble proteins were extracted from agroinfiltrated plant leaves with pBYR2HS-EGFP or pBYR2fp-EGFP. Coomassie Brilliant Blue (CBB) staining and immunoblot analysis with anti-GFP antibodies were performed by using agroinfiltrated leaves of N. benthamiana (A), lettuce (B), eggplants (C), tomatoes (D), hot peppers, and roses (E). The numbers at the top of the gels indicate different samples taken from different leaves from different plants. NT indicates non-transfected plants. The amount of protein was measured according to band intensity from CBB staining gel using ImageJ software. Arrowheads indicate bands corresponding to GFP protein. The band clearly seen at 55 kDa in a CBB staining gel is corresponding to large subunit of Rubisco. Data represent the means ± SD (n = 3 to 4). Significance was determined using unpaired Student’s t tests (*p < 0.05). Full-length gels and full-length blots are presented in Supplementary Figures S1 and S2.