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. 2018 Mar 19;4:44. doi: 10.1038/s41420-018-0045-6

Fig. 4. Decreased expression of E2F1 protein in T cells in response to METH.

Fig. 4

Primary human T were activated with human anti-CD3/CD28 for 48 h treated with METH (100 μM) for 24 h. a Representative immunoblots of E2F1 and internal standard actin of three independent experiments are shown in blots. b The relative fold expression of protein subunits normalized to actin are shown in the histogram. The data are presented as the mean ± SEM of three independent experiments. ∇∇P < 0.001 compared to unactivated cells;**P < 0.001 compared to activated cells