Fig. 7. Gating strategies for the flow cytometry analyses of peripheral blood leukocytes.

Peripheral blood leukocytes from healthy individuals (n = 5) were separated into two broad subsets following staining with cell surface marker CD20, using flow cytometry. Based on their distribution in a side scatter (SS) vs. forward scatter (FS) contour plot, viable leukocytes were selected. B lymphocytes were identified based on their binding of the CD20 antibody, while CD20-negative lymphocytes were identified as T lymphocytes. From this CD20-negative T lymphocytes population, CD4+ and CD8+ T cells were identified based on their binding to CD4 and CD8 antibodies, respectively