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. 2018 Mar 13;9:447. doi: 10.3389/fimmu.2018.00447

Figure 4.

Figure 4

Relation between S100 secretion and neutrophil extracellular trap (NET) formation. (A) Correlation between NETosis and S100A8/A9 secretion. Log values of S100A8/A9 secretion were plotted against log values of NETosis and a two-tailed Pearson correlation test was performed for differentiated HL-60 (dHL-60) cells (left panel) or neutrophils (right panel). Involvement of nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) in NETosis (B) and S100A8/A9 secretion (C) dHL-60 cells (left panel) or neutrophils (right panel) were incubated for 30 min with diphenyleneiodonium (10 μM) then stimulated with phorbol 12-myristoyl 13-acetate (PMA) (100 nM) for 6 h. S100A8/A9 concentration and extracellular NET-DNA were determined in the same condition than in Figures 1 and 3, respectively. Results are presented as mean ± SEM of three (for dHL60) or four (for neutrophils) independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.