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. 2018 Mar 13;9:447. doi: 10.3389/fimmu.2018.00447

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Copyright © 2018 Schenten, Plançon, Jung, Hann, Bueb, Bréchard, Tschirhart and Tolle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine mRNA expression after S100A8/A9 or S100A8/PhosphoA9 stimulation of differentiated HL-60 (dHL-60) cells. dHL-60 cells were stimulated for 30 min, 1, 2, and 4 h with 3 μg/mL of either S100A8/A9 or S100A8/PhosphoA9. Expression of interleukin (IL)-1α (A), IL-1β (B), IL-6 (C), tumor necrosis factor alpha (TNFα) (D), CCL2 (E), CCL3 (F), CCL4 (G), and CXCL8 (H) mRNA were measured by real-time PCR. Results were normalized to three reference genes (Actin-β, B2M, and Gusβ) according to the method of Vandesompele et al. (31) and expressed as fold induction compared to the non-stimulated control (22). Results are presented as mean ± SEM of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.