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. 2018 Mar 13;9:447. doi: 10.3389/fimmu.2018.00447

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Copyright © 2018 Schenten, Plançon, Jung, Hann, Bueb, Bréchard, Tschirhart and Tolle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-dependent cytokine secretion from differentiated HL-60 cells after S100A8/A9 or S100A8/PhosphoA9 stimulation. Cytokine secretion was measured by cytometric bead array upon stimulation with 3 μg/mL S100A8/A9 or S100A8/PhosphoS100A9 for 30 min to 6 h. Tumor necrosis factor alpha (TNFα) (A), interleukin (IL)-6 (B), CCL2 (C), CCL3 (D), CCL4 (E), or CXCL8 (F) concentrations were divided by LDH values in order to correct for cytokine release by cell death. Results are presented as mean ± SEM of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.