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. 2018 Mar 13;9:447. doi: 10.3389/fimmu.2018.00447

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Copyright © 2018 Schenten, Plançon, Jung, Hann, Bueb, Bréchard, Tschirhart and Tolle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR4-dependence of S100A8/PhosphoA9-induced cytokine secretion. Differentiated HL-60 cells were incubated with 1 μg/mL of neutralizing toll-like receptor 4 (TLR4) antibody (aTLR4) or isotype control (IgG) and stimulated with 3 μg/mL S100A8/PhosphoA9 (PS100) for 4 h. Cytokine secretion was measured by cytometric bead array. Concentrations for tumor necrosis factor alpha (TNFα) (A), interleukin (IL)-6 (B), CCL2 (C), CCL3 (D), CCL4 (E), or CXCL8 (F) were divided by LDH values and the results expressed as fold induction of the S100A8/PhosphoA9-stimulated control. Results are presented as mean ± SEM of five independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.