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. 2018 Mar 13;9:445. doi: 10.3389/fmicb.2018.00445

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Copyright © 2018 Wu and Shah.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Correlation analysis of gene expression level from RNA-seq analysis and reverse-transcriptase qPCR assay. Diamond, genes selected from the comparison of condition 2 and condition 1 (pH effect); Circle, genes selected from the comparison of condition 3 and condition 2 (temperature effect); triangle, genes selected from the comparison of condition 4 and condition 2 (WPI effect). The error bar (standard deviation) for -ΔΔC_T (qPCR assay) was indicated; only the mean values for qPCR assay and RNA-seq results were used for linear regression analysis.