Table 1.
Reverse-transcriptase qPCR primers used in this study.
| Comparison group | Gene ID | Gene annotation | Amplicon size (bp) | Forward primer ID | Forward primer (5′ → 3′) | Reverse primer ID | Reverse primer (5′ → 3′) |
|---|---|---|---|---|---|---|---|
| Reference gene | T303_03540 | tuf | 132 | 3450-F | GTGTCCTTCTTCGTGGTATC | 3450-R | GACGTCCACCTTCTTCTTTAG |
| Cd2 vs. Cd1 | T303_00020 | Argininosuccinate lyase | 117 | 20-F | CCAGCTTACCAGCCTTATATTC | 20-R | GCTCTATTGCCCATGTAACC |
| T303_02895 | Cystathionine gamma-synthase | 94 | 2895-F | GGTAAGGGTGGGATGATTTC | 2895-R | GACTCTCCGCAAAGGTAAAG | |
| T303_06345 | epsM | 128 | 6345-F | GTACGATCCAAAGTCACGTATAG | 6345-R | TTACCAGCGAGACCTTACA | |
| T303_06350 | epsL | 123 | 6350-F | TGGCACGAATTTAGGAGTAATAG | 6350-R | GCCTTTGCACATAGAGAGTATAG | |
| T303_08300 | Amino acid transport system substrate-binding protein | 119 | 8300-F | GACTCCTTGGTCTCTTTGATG | 8300-R | ACCCTTGCCAGCTTTAAC | |
| T303_01040 | Tryptophanyl-tRNA synthetase | 138 | 1040-F | CTGGATCTTCCACACGAATATG | 1040-R | TCCCAGGTCTAGATGGTAATG | |
| T303_01340 | Amidophosphoribosyltransferase | 121 | 1340-F | GATTCGTCGTAGCCATAACC | 1340-R | TGGATCCAAAGCTGCAATAA | |
| T303_01945 | CTP synthase | 121 | 1945-F | GCTGGACAAGGAATCAAGAA | 1945-R | TACCCTGAGCCTGCATATTA | |
| T303_07280 | Citrate synthase | 118 | 7280-F | ACACTCATCGGGTGTAAATG | 7280-R | CAACGCAAATGGAACTCAAG | |
| T303_09710 | Glutamine synthetase | 117 | 9710-F | GGAAGTAGTATGCGTCTTGAG | 9710-R | GTATTGCCGGTAGTGGTATG | |
| Cd3 vs. Cd2 | T303_03150 | Fructose operon transcriptional repressor | 97 | 3150-F | CATGCTGTTAAGCTGGTAGA | 3150-R | CCAGAGCTGTACTTCCAATAG |
| T303_05205 | Cell envelope serine proteinase | 94 | 5205-F | AACCACAGTCAGGCAATAAG | 5205-R | CTGTCTGTCCCATTCCATTC | |
| T303_05515 | Glucosamine–fructose-6-phosphate aminotransferase | 111 | 5515-F | CCAGTTGAGCTCGGTATTTC | 5515-R | CTGACGACTATCTGCTGTTTC | |
| T303_07870 | Lactose permease | 100 | 7870-F | TGGCGAGTAGGAAGAATTTG | 7870-R | CTTCAGGTAGCATGGGTAAAG | |
| T303_09015 | Universal stress protein UspA | 95 | 9015-F | GTGCCACTGGTCTCAATAC | 9015-R | ACGAACAACGAGCAAGTC | |
| Cd4 vs. Cd2 | T303_02960 | Cysteine synthase A | 105 | 2960-F | CAGCTGGATTTGAAGGGTTAG | 2960-R | CTGGTAGCGAAGGAATGAAAG |
| T303_03905 | Peptidase M20 | 101 | 3905-F | CCTTCGTTCAGGTGCTATTT | 3905-R | CCGTATCCACACCCAAATC | |
| T303_04075 | Homocysteine S-methyltransferase | 123 | 4075-F | GCTGTAGGCATCAACTGTAG | 4075-R | CTGACTGTCGCCATCATAAA | |
| T303_05970 | O-acetylhomoserine (thiol)-lyase | 135 | 5970-F | TGAGATTGCTCACAGTCATAAG | 5970-R | GTTCCATGTCCACCGATAAA | |
| T303_01480 | Arsenate reductase | 113 | 1480-F | GGTTGAACAAGCATCAACTTC | 1480-R | GATGCTCTCAACACCATTCT |