Table 1.

Model input parameters.

	L-tyrosinea	L-phenylalanineb
Parameters	Value		Unit	References
LAT1 (MBEC)
${{\text{K}}_{\text{m},\text{abs},\text{LAT}1,\text{TL}}}^{\mathrm{\text{P}}(\mathrm{\text{ISF}})}$	64	11	μM	Smith et al., 1987
Vmax,LAT1,lum,TL	0.175	0.075	μmol/min	Smith et al., 1987; Tilgmann et al., 1992
${{\text{K}}_{\text{m},\text{abs},\text{LAT}1,\text{CL}}}^{\mathrm{\text{P(ISF)}}}$	37c	52.9c	μM	Smith et al., 1987
Vmax,LAT1,lum,CL	0.086c	0.129c	μmol/min	Smith et al., 1987; Tilgmann et al., 1992
LAT2 (Astrocyte)
${{\text{K}}_{\text{m},\text{abs},\text{LAT}2,\text{TL}}}^{\text{ISF}(\text{Ast})}$	294d	110.2d	μM	Kim et al., 2004
Vmax,LAT2,TL	0.1128	0.1128	μmol/min	Shank and Campbell, 1984; Segawa et al., 1999
${K_{m,abs,LAT2,CL}}^{\mathrm{\text{ISF(Ast)}}}$	163.6c	185.9c	μM	Kim et al., 2004
Vmax,LAT2,CL	0.1452c	0.1494c	μmol/min	Shank and Campbell, 1984; Segawa et al., 1999
B0AT2 (Neuron)
${{\text{K}}_{\text{m},\text{abs},\text{B}}^0\text{AT}2,\text{TL}}^{\mathrm{\text{ISF(Neu)}}}$	NA	1,050	μM	Bröer et al., 2006
$V_{max,B^{0}AT2,TL}$	NA	0.0086	μmol/min	Rao et al., 1995; Bröer et al., 2006
${{\text{K}}_{\text{m},\text{abs},\text{B}}^0\text{AT}2,\text{CL}}^{\mathrm{\text{ISF(Neu)}}}$	123.5c	126.2c	μM	Bröer et al., 2006
$V_{max,B^{0}AT2,CL}$	0.0184c	0.0186c	μmol/min	Rao et al., 1995; Bröer et al., 2006
${K_{m,B^{0}AT2,Na}}^{\mathrm{\text{ISF(Neu)}}}$	1,050	1,050	μM	Takanaga et al., 2005
ΔΨ	−70	−70	mV	Smith et al., 1981
β	0.6e	0.6e	mV	Takanaga et al., 2005; Panitchob, 2015
[Na]ISF	141	141	mM	Mori et al., 2002
[Na]Neu	40	40	mM	Fedoroff and Vernadakis, 1986
VOLUME
VMBEC	3.5		μl	Mori et al., 2002; Licinio and Wong, 2009
VISF	352.6		μl	Tilgmann et al., 1992; Syková et al., 2005
VAst	742		μl	Ren et al., 1992; Anderova et al., 2011
VNeu	441.7		μl	Ren et al., 1992; Setou et al., 2004; Hosseini-Sharifabad and Nyengaard, 2007

^aIn this column, TL and CL represent L-tyrosine and L-tyrosine competing LNAAs, respectively.

^bIn this column, TL and CL represent L-phenylalanine and L-phenylalanine competing LNAAs, respectively.

^cThe kinetic parameters for the mixture of L-tyrosine and L-phenylalanine competing LNAAs are calculated based on Equation 10 (Supplementary Table 1).

^dThe kinetic parameters are calculated based on Michaelis-Menten equation.

^eEstimated based on data by Takanaga et al. (2005), Figure 7D. NA (not applicable) specifies the large neutral amino acid was not reported to be a substrate for the transporter. For calculation of Vmax values, the total rat brain weight, volume and protein content are considered 1.81 g (Stewart, 1918), 1,737 μl (Tilgmann et al., 1992) and 105 mg protein/g brain (Banay-Schwartz et al., 1992), respectively.