Fig. 2.

BldC binding to the whiI promoter region. a DNase I footprinting analysis. 5′ end-labeled probes were incubated with increasing amounts of BldC (indicated in µM above the lanes) and subjected to DNase I footprinting analysis as described in Methods. Footprints are flanked on the left-hand side by Maxam and Gilbert sequence ladders (AG). Horizontal black arrows indicate sites of enhanced DNase I cleavage on the forward strand and the vertical bar indicates the region of DNase I protection on the reverse strand. b Summary of DNase I footprinting results presented in a. The bracket indicates the protected region and the vertical black arrows indicate sites of enhanced DNase I cleavage. The numbers indicate the distance to the 5′ end of the whiI transcript. The direct repeats shown to be bound by BldC in a head-tail orientation are indicated by arrows (with arrows pointing in the head-tail direction)