Table 1.
Primer sequences of molecular markers developed for nine SWEET genes highly expressed in fruit of apple
| Gene | ID | LG | Gene tag | Primer sequence (5′–3′) | Expected size (bp) | |||
|---|---|---|---|---|---|---|---|---|
| Type | Motif | Location | Forward | Reverse | ||||
| MdSWEET2b | MD11G1270800 | 11 | SSR | (AG)7 | 93 bp of USC | TGAGGCAGAAACAATCATAAGGGTC | GAGCACGGAATTTGAAGCTGTAAAA | 257 |
| MdSWEET2a | MD03G1250600 | 3 | SSR | (GA)11 | 102 bp of USC | ATACCGAGGAACTGTAGGACCAAGC | CTCCACACTAAACAACCAGAAAGCA | 335 |
| MdSWEET2d | MD05G1293100 | 5 | SSR | (AT)8 | 664 bp of USC | CATTCAATTTATTCGACCGGACGAC | TGGGTTCATCCCTCACTTTCACTCA | 270 |
| MdSWEET2e | MD10G1269300 | 10 | SSR | (AT)7 | 4203 bp of USC | GTGAGCCCACAACTAATCCCAT | CTTGTGCGTAGGAATCCCGATA | 219 |
| MdSWEET7b | MD06G1112000 | 6 | SSR | (CT)17 | 192 bp of USC | GGGTTTTGAGAATCTTGAGGGTAGG | TTTGATGGGTTGGACTGTAACTTGC | 251 |
| MdSWEET9b | MD04G1236000 | 4 | SSR | (CT)19 | Second intron | GCGCCAATGTAAGACCCTTTACTTT | CTGACCTTGTCCTTCTTGGATGCGTA | 348 |
| MdSWEET7a | MD11G1299200 | 11 | SSR | (CT)14 | Second intron | TTCTATCTCCCCTTCCCAAACTTCC | GCTAAACAGTGCCACTGCATAAGGT | 340 |
| MdSWEET12a | MD14G1151300 | 14 | SSR | (TA)10 | 1902 bp of USC | ATGACAGGGCAACTTCAGGGT | CGTAATAGTCCTTTGCCCTCC | 253 |
| MdSWEET15a | MD16G1125300 | 16 | CAPS | T/C | First intron | ACCTACCAATCCTCCATCTGTC | CCATCACAATAACTCACCTGCTTC | 641 |
USC upstream of start codon